Title: antibacterial activity of aqueous and ethanolic extracts of root and stem bark of Bridelia ferruginea. Benth. (Euphorbiaceae)
Author(s): Mela Ilu Luka, Stanley Chukwudozie Onuoha, Vincent Olasoji Oladele and John Aguiyi
Year 2020
Publisher: Journal of Medicinal Plants Research
File: PDF
Keywords: Antibacterial phytochemical screening Bridelia ferruginea plant extracts.
This study aimed at analysing the phytochemical content and antimicrobial activity of Bridelia ferrugine
against selected bacteria. Total saponin, alkaloids, tannins, flavonoids and total anthraquinone
contents were evaluated using spectrophotometric equivalents of the standards. The antibacterial
activity of the plant extracts were determined using Minimum Inhibitory Concentration (MIC) and
Minimum Bactericidal Concentration (MBC) assays against selected bacteria. The root and stem
revealed the presence of the phytochemicals tested except the stem that lacked anthraquinones. In
vitro antimicrobial activity of the plant extracts against the gram-positive bacteria tested showed that
Bacillus cereus was most susceptible to the plant extract having MIC and MBC of 25 and 50 mg/ml,
respectively for the stem-bark and root-bark ethanolic extract, while gram-negative bacteria the plant
extracts were most active against Proteus mirabilis with MIC and MBC of 50 and 100 mg/ml,
respectively. The aqueous extract was most active against Staphylococcus epidermidis with MIC and
MBC of 50 and 100 mg/ml for stem-bark and 25 and 50 mg/ml for root-bark extract. Concentration
dependent study showed the plant extracts were either bacteriostatic or bactericidal. Only the stembark
aqueous extract showed no primary effect on the control strains. The study confirmed the
presence of some phytochemicals which revealed that the plant is of pharmacological importance
going by the ability of these phytochemicals to elicit antibacterial activity.